| 质粒名称: | pcDNA3.4_7C11-1 light chain |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 198252 |
| 存储实验室: | Aymelt Itzen |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.4 |
| 载体出品公司: | Clontech |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | 6712bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
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| 插入基因名称: | light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa) / 7C11 LC |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 759 bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | JN247409 |
| 启动子: | CMV |
| 标签/融合蛋白: | IL10 signal peptide (N terminal on insert) |
| 克隆方法: | 未知 |
| 5’测序引物 | CGCAAATGGGCGGTAGGCGTG |
| 3’测序引物 | CATAGCGTAAAAGGAGCAACA |
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文献引用方法 |
|
| 材料和方法部分: |
pcDNA3.4_7C11-1 light chain was a gift from Aymelt Itzen (Addgene plasmid # 198252 ; http://n2t.net/addgene:198252 ; RRID:Addgene_198252)
|
| 参考文献部分: | Monoclonal Anti-AMP Antibodies Are Sensitive and Valuable Tools for Detecting Patterns of AMPylation. Hopfner D, Fauser J, Kaspers MS, Pett C, Hedberg C, Itzen A. iScience. 2020 Nov 13;23(12):101800. doi: 10.1016/j.isci.2020.101800. eCollection 2020 Dec 18. 10.1016/j.isci.2020.101800 PubMed 33299971 |
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